Free Fatty Acid (FFA) Content Assay Kit
UPLC-MS-4407_游离脂肪酸含量(FFA)试剂盒-测植物组织
UPLC-MS-4407
100管/96样
96 Sample
按照标签要求储存
产品参数
酶标仪
酶标仪/96孔板
5 h (100 Sample)
正庚烷(C7H16,MW=100.2,CAS: 142-82-5);
铜皂法
550 nm
递增型
Palmitic Acid
y=0.6659x-0.0145 (R²=0.999)
0.1-2.0 µmol/mL
0.04 µmol/mL
4℃ Wet Ice Transportation
检测原理
游离脂肪酸与铜离子结合可形成脂肪酸铜,铜离子与显色液反应形成紫红色络合物,产物在550 nm处具有特征吸收峰,通过吸光值变化即可定量检测游离脂肪酸的含量。
产品标准注意事项
①试剂二配制应尽量晚配,可在操作到加入试剂一时,再配制试剂二;
实验注意事项
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
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