Alcohol Dehydrogenase (ADH) Activity Assa
UPLC-MS-4341_乙醇脱氢酶(ADH)试剂盒
UPLC-MS-4341
110管/100样
100 Samples
按照标签要求储存
产品参数
酶标仪
酶标仪/96孔UV板
5 h (100 Samples)
NADH速率法
NADH速率法
340 nm
递减型
-20℃ Dry Ice Transportation
检测原理
乙醇脱氢酶能够催化乙醛生成乙醇,并将NADH氧化为NAD+,NADH在340 nm具有特征吸收峰,通过吸光值变化即可表征乙醇脱氢酶的活性。
产品标准注意事项
①准确在17 s和75 s处完成读数,以保证实验结果的准确性;若使用96孔UV板需使用多道移液器,且分批进行检测,以保证组间反应时间统一;
实验注意事项
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
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