β-1,3-Glucanase (β-1,3-GA) Activity Ass
UPLC-MS-4216_β-1,3葡聚糖酶(β-1,3-GA)试剂盒
UPLC-MS-4216
50管/24样
24 Sample
按照标签要求储存
产品参数
可见分光光度计
可见分光光度计/1 mL玻璃比色皿(d=10 mm)
3 h (25 Sample)
DNS比色法
540 nm
递增型
Glucose
To Be Updated
0.2-1.0 μmol/mL
To Be Updated
4℃ Wet Ice Transportation
检测原理
β-1,3葡聚糖酶能够内切昆布多糖中的β-1,3-葡萄糖苷键产生还原末端,与DNS生成红棕色物质,产物在540 nm有特征吸收峰,通过吸光值的变化即可表征β-1,3葡聚糖酶的活性。
产品标准注意事项
①若ΔA测定超出标准线性吸光值范围,建议适当增加样本量或扩大提取液的稀释倍数后再进行测定,计算时相应修改;
实验注意事项
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
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